Difference between revisions of "Part:BBa K2271115"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | You can use an alcohol dehydrogenase(ADH) as part of the nootkatone pathway. This ADH is from Pichia pastoris. | |
− | It converts (+)-trans-nootkatol into (+)-nootkatone by oxidation, using NAD+ as a cofactor. | + | It converts (+)-trans-nootkatol into (+)-nootkatone by oxidation, using NAD+ as a cofactor. Regarding the cofactorregeneration one can use BM3 or an other enzyme to convert NADH+H+ into NAD+. |
In nature ADH reduces or oxidizes alcohols to their specific aldehydes or ketones with help of NAD+. | In nature ADH reduces or oxidizes alcohols to their specific aldehydes or ketones with help of NAD+. | ||
Latest revision as of 03:54, 2 November 2017
ADH alcohol dehydrogenase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 2047
Illegal BglII site found at 880 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2402
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
You can use an alcohol dehydrogenase(ADH) as part of the nootkatone pathway. This ADH is from Pichia pastoris. It converts (+)-trans-nootkatol into (+)-nootkatone by oxidation, using NAD+ as a cofactor. Regarding the cofactorregeneration one can use BM3 or an other enzyme to convert NADH+H+ into NAD+. In nature ADH reduces or oxidizes alcohols to their specific aldehydes or ketones with help of NAD+.
This part is designed for cytosolic expression, for peroxisomal import please choose part: BBa_K2271116