Difference between revisions of "Part:BBa K2510112"

 
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<partinfo>BBa_K2510112 short</partinfo>
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<partinfo>BBa_K2510108 short</partinfo>
  
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<p> This part encodes for TetR in wtdronpa </p>
  
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<p> In our project we developed a logic gate at the promoter level by creating dually repressed promoters using different combinations of the operators for TetR, P22 c2 and HK CI, the logic gate was tested with Dronpa-repressors fusions, the fluorescence levels increased over 1000-fold when violet light is shone    </p>
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<img src="https://static.igem.org/mediawiki/2017/e/e2/Logic_tetR_PB.png">
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<img src="https://static.igem.org/mediawiki/2017/8/8e/Logic_P22c2_PB.png">
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<img src="https://static.igem.org/mediawiki/2017/1/19/Logic_HKcIfigure_PB.png">
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                                            <span  class="image-span text-center">
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                                            <b>Figure 1:Results of the cell-free experiment. Each promoter was tested with its cognate repressors. Top: Testing with TetR caged with either wt-Dronpa (BBa_K2510108) or a mutated version(BBa_K2510109)Middle: Testing with P22c2 caged with either wt-Dronpa (BBa_K2510112) or a mutated version(BBa_K2510113).Bottom: Testing with HKcI caged with either wt-Dronpa (BBa_K2510110) or a mutated version(BBa_K2510111)
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2510108 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2510112 parameters</partinfo>
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<partinfo>BBa_K2510108 parameters</partinfo>
 
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Revision as of 03:54, 2 November 2017


pdDronpa wt TetR

This part encodes for TetR in wtdronpa

In our project we developed a logic gate at the promoter level by creating dually repressed promoters using different combinations of the operators for TetR, P22 c2 and HK CI, the logic gate was tested with Dronpa-repressors fusions, the fluorescence levels increased over 1000-fold when violet light is shone




<img src="Logic_tetR_PB.png"> <img src="Logic_P22c2_PB.png"> <img src="Logic_HKcIfigure_PB.png"> 

                                           
                                           Figure 1:Results of the cell-free experiment. Each promoter was tested with its cognate repressors. Top: Testing with TetR caged with either wt-Dronpa (BBa_K2510108) or a mutated version(BBa_K2510109)Middle: Testing with P22c2 caged with either wt-Dronpa (BBa_K2510112) or a mutated version(BBa_K2510113).Bottom: Testing with HKcI caged with either wt-Dronpa (BBa_K2510110) or a mutated version(BBa_K2510111)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]