Difference between revisions of "Part:BBa K1724002"
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The quantification data of MerR/CadA operon can be seen in BBa_K1724000.[[https://parts.igem.org/Part:BBa_K1724000]] | The quantification data of MerR/CadA operon can be seen in BBa_K1724000.[[https://parts.igem.org/Part:BBa_K1724000]] | ||
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| + | IMPROVEMENT: | ||
| + | iGEM Peshawar 2017 has gotten this part synthesized and has submitted it to the registry for future use by teams. | ||
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Revision as of 03:54, 2 November 2017
MerR
The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.
The quantification data of MerR/CadA operon can be seen in BBa_K1724000.[[1]]
IMPROVEMENT: iGEM Peshawar 2017 has gotten this part synthesized and has submitted it to the registry for future use by teams.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 258
Illegal AgeI site found at 360 - 1000COMPATIBLE WITH RFC[1000]