Difference between revisions of "Part:BBa K2194003"

Latest revision as of 03:53, 2 November 2017

cysPUWA Sulfate Transport System cds

The cysPUWA genes encode the sulfate ABC membrane transporter in Escherichia coli. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for cysP, cysU, cysW, and cysA in the context in which they appear in E. coli MG1655.

Biology and Usage

The binding protein cysP increases the affinity for sulfate uptake; genes cysU and cysW encode the integral membrane proteins. The final cds in this BioBrick is for cysA which is an ATP-binding subunit.

Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI). The lower growth rate for cysPUWA transformants in the presence of Cr(VI) indicates the higher rate of chromium import and resulting toxicity.

Figure 1: Effect of Chromate on E. coli Growth

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1274
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2090
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3
Illegal BsaI.rc site found at 3828

@@ Line 7: / Line 7: @@
 ===Biology and Usage===
-Cys P is a binding protein which increases the affinity for sulfate uptake;  genes cysU, cysW encode the integral membrane proteins. cysA is an ATP-binding subunit.
+The binding protein <i>cysP</i> increases the affinity for sulfate uptake; genes <i>cysU</i> and <i>cysW</i> encode the integral membrane proteins. The final cds in this BioBrick is for <i>cysA</i> which is an ATP-binding subunit.
-Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI): lower growth rate for cysPUWA transformants in the presence of Cr(VI) may indicate the higher rate of chromium transport inside the cell.
+Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI). The lower growth rate for cysPUWA transformants in the presence of Cr(VI) indicates the higher rate of chromium import and resulting toxicity.