Difference between revisions of "Part:BBa K2260001:Design"
 
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  The phaC1J4 operon was codon optimized for use in E. coli using the <a href ="http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606"> codon optimization table</a>. Lastly, histidine tags were added to the operon.
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  The phaC1J4 operon was codon optimized for use in <i>E. coli</i> using <a href ="http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606">this</a> codon optimization table. histidine tags were added to the N-termini of each protein in the operon for protein purification.
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Latest revision as of 03:51, 2 November 2017

phaC1J4 w/ Histidine tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The phaC1J4 operon was codon optimized for use in E. coli using this codon optimization table. histidine tags were added to the N-termini of each protein in the operon for protein purification.

Source

The phaC1 was obtained from Pseudomonas aeruginosa, whereas phaJ4 was obtained from Pseudomonas putida.

References