Difference between revisions of "Part:BBa K2500013"
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<p>In the absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the operator sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcriptional activation by the quorum sensing components.</p>
 
<p>In the absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the operator sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcriptional activation by the quorum sensing components.</p>
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  <img src="https://static.igem.org/mediawiki/2017/1/13/T--ETH_Zurich--expressioncassette.png" width="50%">
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<p>This part is the regulator cassette of the AND-gates <a href="https://parts.igem.org/Part:BBa_K2500010">BBa_K2500010</a>, <a href="https://parts.igem.org/Part:BBa_K2500011">BBa_K2500011</a>, <a href="https://parts.igem.org/Part:BBa_K2500010">BBa_K2500012</a>.</p>
 
<p>This part is the regulator cassette of the AND-gates <a href="https://parts.igem.org/Part:BBa_K2500010">BBa_K2500010</a>, <a href="https://parts.igem.org/Part:BBa_K2500011">BBa_K2500011</a>, <a href="https://parts.igem.org/Part:BBa_K2500010">BBa_K2500012</a>.</p>

Revision as of 03:47, 2 November 2017

Expression cassette of LuxR and LldP/LldR

The AND gate integrates two signals, namely the presence of high L-lactate concentrations and high bacterial cell density (qorum sensing) and regulates the effector functions of CATE. It allows our engineered bacteria to autonomously decide whether they are currently located in tumor tissue or not.

In the absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the operator sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcriptional activation by the quorum sensing components.

This part is the regulator cassette of the AND-gates BBa_K2500010, BBa_K2500011, BBa_K2500012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2507
    Illegal NheI site found at 2530
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 334
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 470
    Illegal AgeI site found at 933
    Illegal AgeI site found at 1371
    Illegal AgeI site found at 1530
    Illegal AgeI site found at 1542
    Illegal AgeI site found at 2281
  • 1000
    COMPATIBLE WITH RFC[1000]