Difference between revisions of "Part:BBa K2398023"

Latest revision as of 03:36, 2 November 2017

M13 bacteriophage backbone w/o gene III for application in Phage-assisted continous evolution (PACE)

This part is directly related to our Phage-assisted continous evolution (PACE) and Phage-related discontinous evolution (PREDCEL) Toolbox. This part can be used directly for the generation of geneIII-deficient M13 bacteriophages. For further information on how to use these parts, please visit our Toolbox guide (http://2017.igem.org/Team:Heidelberg/Toolbox) and our RFC (http://2017.igem.org/Team:Heidelberg/RFC. Important information on how to finally produce bacteriophages can also be found the method section (http://2017.igem.org/Team:Heidelberg/Experiments). Since this part contains a kanamycine resistance, the bacteriophages can potentially be selected by addition of kanamycine to the medium after successfully performed transformation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 5379
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2971
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2
Illegal BsaI.rc site found at 6190

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2398023 short</partinfo>
-TO DO
+This part is directly related to our Phage-assisted continous evolution (PACE) and Phage-related discontinous evolution (PREDCEL) Toolbox. This part can be used directly for the generation of geneIII-deficient M13 bacteriophages. For further information on how to use these parts, please visit our Toolbox guide (http://2017.igem.org/Team:Heidelberg/Toolbox) and our RFC (http://2017.igem.org/Team:Heidelberg/RFC. Important information on how to finally produce bacteriophages can also be found the method section (http://2017.igem.org/Team:Heidelberg/Experiments). Since this part contains a kanamycine resistance, the bacteriophages can potentially be selected by addition of kanamycine to the medium after successfully performed transformation.
 <!-- Add more about the biology of this part here