Difference between revisions of "Part:BBa K2510101"

Latest revision as of 03:32, 2 November 2017

PDDronpa wt t7 promoter

This part is the same exact dronpa version as the part Part:BBa K2510100, except that it has T7 promoter and lac operator.

Dronpa is a reversible photoswitchable fluorescent protein that is switched on by default “fluorescent” and is switched off when illuminated by cyan light (~500nm). Dronpa Fluorescence is recovered by shining violet light (~400nm). And has been used in a design that facilitates the optical control of protein activities This part contains a device of two copies of Dronpa Fluorescent Protein that are codon optimized for E Coli with two BsaI cutting site in between to allow the insertion of various proteins to be tested </p? Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 8
Illegal NheI site found at 31
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 805
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 808
Illegal BsaI.rc site found at 802

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 <partinfo>BBa_K2510101 short</partinfo>
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+<p>This part is the same exact dronpa version as the part Part:BBa K2510100, except that it has T7 promoter and lac operator.</p>
+ <p>Dronpa is a reversible photoswitchable fluorescent protein that is switched on by default “fluorescent” and is switched off when illuminated by cyan light (~500nm). Dronpa Fluorescence is recovered by shining violet light (~400nm). And has been used in a design that facilitates the optical control of protein activities This part contains a device of two copies of Dronpa Fluorescent Protein that are codon optimized for E Coli with two BsaI cutting site in between to allow the insertion of various proteins to be tested </p?
 <!-- Add more about the biology of this part here