Difference between revisions of "Part:BBa K2381023"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2381023 short</partinfo>
 
<partinfo>BBa_K2381023 short</partinfo>
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<p>It’s a gRNA sequence containing a light-induced promoter, 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold.
 
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It's function is blocking the replication initiation.
 
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[[File:T--HZAU-China--light_gRNA.png|200px|thumb|left|Its function is proved by OD measurement.]] </p>
 
  
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<p>References<br/>
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<html>
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1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.<br/>
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<body>
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2. Schmidl, S. R., Sheth, R. U., Wu, A., & Tabor, J. J. (2014). Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol, 3(11), 820-831.
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<p>
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It’s a gRNA sequence containing a light-induced promoter, 20bp targeting at oriC and a full-length gRNA scaffold. It's function is blocking the replication initiation.
 
</p>
 
</p>
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<img src="https://static.igem.org/mediawiki/parts/f/f1/T--HZAU-China--light_gRNA.png" width="700px"/>
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</br>
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Its function is proved by OD measurement.
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</br>
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</br>
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</br>
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<h3>References</h3>
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1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.
 +
2. Schmidl, S. R., Sheth, R. U., Wu, A., & Tabor, J. J. (2014). Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol, 3(11), 820-831.
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</body>
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:32, 2 November 2017


PcpcG2-gRNA (light-control cell replication inhibitor)

It’s a gRNA sequence containing a light-induced promoter, 20bp targeting at oriC and a full-length gRNA scaffold. It's function is blocking the replication initiation.


Its function is proved by OD measurement.


References

1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810. 2. Schmidl, S. R., Sheth, R. U., Wu, A., & Tabor, J. J. (2014). Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol, 3(11), 820-831.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]