Difference between revisions of "Part:BBa K2414003"

Latest revision as of 03:30, 2 November 2017

phytase with cysteine module

This part is composed of phytase with self-assembling region and cysteine module under lac promoter.

HokkaidoU_Japan 2017 analyzed this part. As temperature rises, this phytase's activity falls as below graph. Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer Digest phytic acid in above situation with sample phytase solution 10μl, 37degrees celcius, 10 min. After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1585
Illegal BglII site found at 1639
Illegal BamHI site found at 352
Illegal BamHI site found at 1543
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 418
Illegal NgoMIV site found at 819
Illegal AgeI site found at 1502
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1311

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2414003 short</partinfo>
-This part is composed of  lactose inhibitor under constitutive promoter and phytase with cysteine module under lac promoter.
+This part is composed of  phytase with self-assembling region and cysteine module under lac promoter.
 HokkaidoU_Japan 2017 analyzed this part. As temperature rises, this phytase's activity falls as below graph. Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer Digest phytic acid in above situation with sample phytase solution 10μl, 37degrees celcius, 10 min. After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes.