Difference between revisions of "Part:BBa K2414002"
Ykishimotoy (Talk | contribs) |
Ykishimotoy (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2414002 short</partinfo> | <partinfo>BBa_K2414002 short</partinfo> | ||
| − | This part is composed of | + | This part is composed of phytase with self-assembling region and cysteine module under lac promoter. |
HokkaidoU_Japan 2017 analyzed this part. As temperature rises, this phytase's activity falls as below graph. | HokkaidoU_Japan 2017 analyzed this part. As temperature rises, this phytase's activity falls as below graph. | ||
Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer | Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer | ||
Latest revision as of 03:29, 2 November 2017
phytase with self-assembling region and cysteine module
This part is composed of phytase with self-assembling region and cysteine module under lac promoter. HokkaidoU_Japan 2017 analyzed this part. As temperature rises, this phytase's activity falls as below graph. Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer Digest phytic acid in above situation with sample phytase solution 10μl, 37degrees celcius, 10 min. After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1585
Illegal BglII site found at 1639
Illegal BamHI site found at 352
Illegal BamHI site found at 1543 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 418
Illegal NgoMIV site found at 819
Illegal AgeI site found at 1502 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1311