Difference between revisions of "Part:BBa K2381019"
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this part can response to blue light (470nm), rapidly bind to the corresponding parts, BBa_K2381013, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece. | this part can response to blue light (470nm), rapidly bind to the corresponding parts, BBa_K2381013, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece. | ||
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| − | <img src="https://static.igem.org/mediawiki/parts/d/df/HZAU_2017_MagLuc.png" width=" | + | <img src="https://static.igem.org/mediawiki/parts/d/df/HZAU_2017_MagLuc.png" width="600px"/> |
</br> | </br> | ||
| − | <b>Fig.1 </b>Prove the function of pMag and nMag | + | <b>Fig.1 </b>Prove the function of pMag and nMag. n=1 |
</br> | </br> | ||
</br> | </br> | ||
Revision as of 03:28, 2 November 2017
light-activated split Luciferase
tthe light-activated split Luciferase, used to verify the function of light-induced dimerization protein. this part can response to blue light (470nm), rapidly bind to the corresponding parts, BBa_K2381013, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.
Fig.1 Prove the function of pMag and nMag. n=1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2595
Illegal AgeI site found at 1442
Illegal AgeI site found at 2734 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2236