Difference between revisions of "Part:BBa K2442400"
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<partinfo>BBa_K2442400 short</partinfo> | <partinfo>BBa_K2442400 short</partinfo> | ||
| + | ====This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.==== | ||
[[Image:Glasgow_gfp_gate.png|450px|thumb|center|A schematic representation of a final split-GFP AND-gate]] | [[Image:Glasgow_gfp_gate.png|450px|thumb|center|A schematic representation of a final split-GFP AND-gate]] | ||
Latest revision as of 03:28, 2 November 2017
B0032 + GFP split C-term
This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.
This is a composite of the GFP C-term split subunit (GFP2) K2442397 and the well characterised "weak" ribosome binding site B0032.
Usage and Biology
Despite the sequence error in the original K2442397 part, this composite was used for assembly of larger composite split-GFP AND-gate constructs.
- K2442406 [split GFP module 2] lacI regulated promoter + RBS + GFP C-term + double terminator
- K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 195