Difference between revisions of "Part:BBa K2398021"

Revision as of 03:25, 2 November 2017

M13 bacteriophage backbone part 1 w/o gene III for application in Phage-assisted continous evolution

This part is directly related to our Phage-assisted continous evolution (PACE) and Phage-related discontinous evolution (PREDCEL) Toolbox. Together with https://parts.igem.org/Part:BBa_K2398022 this part is needed for the generation of geneIII-deficient M13 bacteriophages. For further information on how to use these parts, please visit our Toolbox guide (http://2017.igem.org/Team:Heidelberg/Toolbox) and our RFC (http://2017.igem.org/Team:Heidelberg/RFC. Important information on how to finally produce bacteriophages can also be found the method section (http://2017.igem.org/Team:Heidelberg/Experiments).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 110
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2
Illegal BsaI.rc site found at 2690

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2398021 short</partinfo>
-This part is directly related to our Phage-assisted continous evolution (PACE) and Phage-related discontinous evolution (PREDCEL) Toolbox. Together with https://parts.igem.org/Part:BBa_K2398022 this part is needed for the generation of geneIII-deficient bacteriophages. For further use of the backbone parts, please visit our Toolbox guide (http://2017.igem.org/Team:Heidelberg/Toolbox) and our RFC (http://2017.igem.org/Team:Heidelberg/RFC)for proper usage and important aspects for golden gate assembly with various genes of interest.
+This part is directly related to our Phage-assisted continous evolution (PACE) and Phage-related discontinous evolution (PREDCEL) Toolbox. Together with https://parts.igem.org/Part:BBa_K2398022 this part is needed for the generation of geneIII-deficient M13 bacteriophages. For further information on how to use these parts, please visit our Toolbox guide (http://2017.igem.org/Team:Heidelberg/Toolbox) and our RFC (http://2017.igem.org/Team:Heidelberg/RFC. Important information on how to finally produce bacteriophages can also be found the method section (http://2017.igem.org/Team:Heidelberg/Experiments).
 <!-- Add more about the biology of this part here