Difference between revisions of "Part:BBa K2350014:Design"
(→References) |
(→References) |
||
Line 35: | Line 35: | ||
===References=== | ===References=== | ||
− | Cunningham, F. X., Sun, Z., Chamovitz, D., Hirschberg, J., & Gantt, E. (1994). Molecular structure and enzymatic function of lycopene cyclase from the cyanobacterium Synechococcus sp strain PCC7942. The Plant Cell, 6(8), 1107–1121. | + | 1. Cunningham, F. X., Sun, Z., Chamovitz, D., Hirschberg, J., & Gantt, E. (1994). Molecular structure and enzymatic function of lycopene cyclase from the cyanobacterium Synechococcus sp strain PCC7942. The Plant Cell, 6(8), 1107–1121. |
<br> | <br> | ||
− | Chen, P. H., Liu, H. L., Chen, Y. J., Cheng, Y. H., Lin, W. L., Yeh, C. H., & Chang, C. H. (2012). Enhancing CO 2 bio-mitigation by genetic engineering of cyanobacteria. Energy & Environmental Science, 5(8), 8318-8327. | + | 2. Chen, P. H., Liu, H. L., Chen, Y. J., Cheng, Y. H., Lin, W. L., Yeh, C. H., & Chang, C. H. (2012). Enhancing CO 2 bio-mitigation by genetic engineering of cyanobacteria. Energy & Environmental Science, 5(8), 8318-8327. |
<br> | <br> | ||
− | Jacobus, A. P., & Gross, J. (2015). Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli. PLoS ONE, 10(3), e0119221. | + | 3. Jacobus, A. P., & Gross, J. (2015). Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli. PLoS ONE, 10(3), e0119221. |
Latest revision as of 03:24, 2 November 2017
Design Notes
PCR primers for backbone pPIGBACK (BBa_K2350009) to get Ampicillin resistance gene which has been processed a PstI site-directed mutagenesis
Forward: TGCGCTCTCGCTAGTGGTCAcgcggaacccctatttgttt
Reverse: CACCCGCTGCAAAGCACGATttaccaatgcttaatcagtg
PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase upstream
Forward: CAATATCCCGTAAAGTTTTT
Reverse: aaacaaataggggttccgcgTGACCACTAGCGAGAGCGCA
PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase downstream
Forward: cactgattaagcattggtaaATCGTGCTTTGCAGCGGGTG
Reverse: CGGCGTTACCGTCACGTAGT
NOTE:
In order to process a 3 pieces fusion PCR, the PCR primers for Lycopene cyclase upstream and downstream are different from the primers showed in BBa_K2350007 and BBa_K2350008.
Source
Synechoccocus elongatus PCC7942 genomic DNA
Backbone pPIGBACK (BBa_K2350009)
References
1. Cunningham, F. X., Sun, Z., Chamovitz, D., Hirschberg, J., & Gantt, E. (1994). Molecular structure and enzymatic function of lycopene cyclase from the cyanobacterium Synechococcus sp strain PCC7942. The Plant Cell, 6(8), 1107–1121.
2. Chen, P. H., Liu, H. L., Chen, Y. J., Cheng, Y. H., Lin, W. L., Yeh, C. H., & Chang, C. H. (2012). Enhancing CO 2 bio-mitigation by genetic engineering of cyanobacteria. Energy & Environmental Science, 5(8), 8318-8327.
3. Jacobus, A. P., & Gross, J. (2015). Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli. PLoS ONE, 10(3), e0119221.