Difference between revisions of "Part:BBa K2194004"
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Refer to the registry pages for <i>PgntK</i> ([https://parts.igem.org/Part:BBa_K2194002  BBa_K2194002]), a synthetic RBS, cysPUWA proteins ([https://parts.igem.org/Part:BBa_K2194003  BBa_K2194003]), Elowitz RBS ([https://parts.igem.org/Part:BBa_B0034  BBa_B0034]), sulfate binding protein sbp ([https://parts.igem.org/Part:BBa_K2194001  BBa_K2194001]) for detailed biology.  
 
Refer to the registry pages for <i>PgntK</i> ([https://parts.igem.org/Part:BBa_K2194002  BBa_K2194002]), a synthetic RBS, cysPUWA proteins ([https://parts.igem.org/Part:BBa_K2194003  BBa_K2194003]), Elowitz RBS ([https://parts.igem.org/Part:BBa_B0034  BBa_B0034]), sulfate binding protein sbp ([https://parts.igem.org/Part:BBa_K2194001  BBa_K2194001]) for detailed biology.  
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Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI): lower growth rate for cysPUWA transformants in the presence of Cr(VI) may indicate the higher rate of chromium transport inside the cell.
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[[File:Cys_growth.png|600px]]
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<p class = "caption"> <b> Figure 1:</b> Effect of Chromate on <i>E. coli</i> Growth </p>
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Revision as of 03:20, 2 November 2017


Sulfate Transport System w/ Negative Feedback for Membrane Stress

This transcriptional unit codes for the genetic parts necessary to bind and transport chromate, sulfate, and related species across the cell membrane. BioBrick BBa_K2194004 is a composite part composed of negative feedback membrane stress promoter PgntK (BBa_K2194002), a synthetic RBS, cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), sulfate binding protein sbp (BBa_K2194001), and a synthetic terminator.

Biology and Usage

Refer to the registry pages for PgntK (BBa_K2194002), a synthetic RBS, cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), sulfate binding protein sbp (BBa_K2194001) for detailed biology.

Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI): lower growth rate for cysPUWA transformants in the presence of Cr(VI) may indicate the higher rate of chromium transport inside the cell.


Cys growth.png

Figure 1: Effect of Chromate on E. coli Growth



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2296
  • 1000
    COMPATIBLE WITH RFC[1000]