Difference between revisions of "Part:BBa K2522002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | This part is an improvement on the Part BBa_K117008. It functions to sense AI-2 still, but YFP is replaced with Super Folder Green Fluorescent Protein (sfGFP), a protein that glows much quicker (around 4 hours to become visible) and is more useful for a diagnostic approach. This plasmid was created from ligation using New England Biolabs products and protocols. | |
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===Source=== | ===Source=== |
Latest revision as of 03:18, 2 November 2017
sfGFP expressed in the presence of AI-2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 141
Design Notes
This part is an improvement on the Part BBa_K117008. It functions to sense AI-2 still, but YFP is replaced with Super Folder Green Fluorescent Protein (sfGFP), a protein that glows much quicker (around 4 hours to become visible) and is more useful for a diagnostic approach. This plasmid was created from ligation using New England Biolabs products and protocols.
Source
Backbone acquired from parts kit and sfGFP insert from another lab