Difference between revisions of "Part:BBa K2379004:Design"

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===Design Notes===
 
===Design Notes===
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This part has been obtained by replacing the Shine Dalgarno sequence of E coli to that of Bacillus Subtilis, present in the RNA Thermometer BBa_K115001. The sequence was fed to RNA VIENNA FOLD and it was found that the structure of the RNA thermometer for Bacillus Subtilis was very similar to that of E Coli with nearly equal thermodynamic free energies.
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This part has been obtained by replacing the Shine Dalgarno sequence of E coli to that of Bacillus Subtilis, present in the RNA Thermometer[https://parts.igem.org/Part:BBa_K2379001#BBa_K2379001 BBa_K2379001](BBa_K2379001). The sequence was fed to RNA VIENNA FOLD and it was found that the structure of the RNA thermometer for Bacillus Subtilis was very similar to that of E Coli with nearly equal thermodynamic free energies.
  
 
===Source===
 
===Source===

Latest revision as of 03:17, 2 November 2017


RNA Thermometer Optimised for Bacillus Subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been obtained by replacing the Shine Dalgarno sequence of E coli to that of Bacillus Subtilis, present in the RNA ThermometerBBa_K2379001(BBa_K2379001). The sequence was fed to RNA VIENNA FOLD and it was found that the structure of the RNA thermometer for Bacillus Subtilis was very similar to that of E Coli with nearly equal thermodynamic free energies.

Source

nil

References

1. Band, L. and Henner,D. J.: Bacillus subtilis requires a “stringent” Shine-Dalgarno region for gene expression.DNA 3 (1984) 17-21.