Difference between revisions of "Part:BBa K2398021"

 
Line 3: Line 3:
 
<partinfo>BBa_K2398021 short</partinfo>
 
<partinfo>BBa_K2398021 short</partinfo>
  
−
TO DO
+
This part is directly related to our Phage-assisted continous evolution (PACE) and Phage-related discontinous evolution (PREDCEL) Toolbox. Together with https://parts.igem.org/Part:BBa_K2398022 this part is needed for the generation of geneIII-deficient bacteriophages. For further use of the backbone parts, please visit our Toolbox guide (http://2017.igem.org/Team:Heidelberg/Toolbox) and our RFC (http://2017.igem.org/Team:Heidelberg/RFC)for proper usage and important aspects for golden gate assembly with various genes of interest.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:16, 2 November 2017


M13 bacteriophage backbone part 1 w/o gene III for application in Phage-assisted continous evolution

This part is directly related to our Phage-assisted continous evolution (PACE) and Phage-related discontinous evolution (PREDCEL) Toolbox. Together with https://parts.igem.org/Part:BBa_K2398022 this part is needed for the generation of geneIII-deficient bacteriophages. For further use of the backbone parts, please visit our Toolbox guide (http://2017.igem.org/Team:Heidelberg/Toolbox) and our RFC (http://2017.igem.org/Team:Heidelberg/RFC)for proper usage and important aspects for golden gate assembly with various genes of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2
    Illegal BsaI.rc site found at 2690