Difference between revisions of "Part:BBa K2350014:Design"
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PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase upstream | PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase upstream | ||
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| − | Forward: | + | Forward: CAATATCCCGTAAAGTTTTT |
<br> | <br> | ||
| − | Reverse: | + | Reverse: aaacaaataggggttccgcgTGACCACTAGCGAGAGCGCA |
<br> | <br> | ||
PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase downstream | PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase downstream | ||
<br> | <br> | ||
| − | Forward: | + | Forward: cactgattaagcattggtaaATCGTGCTTTGCAGCGGGTG |
<br> | <br> | ||
| − | Reverse: | + | Reverse: CGGCGTTACCGTCACGTAGT |
<br> | <br> | ||
| − | NOTE: | + | NOTE: |
<br> | <br> | ||
| + | In order to process a 3 pieces fusion PCR, the PCR primers for Lycopene cyclase upstream and downstream are different from the primers showed in BBa_K2350007 and BBa_K2350008. | ||
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===Source=== | ===Source=== | ||
Synechoccocus elongatus PCC7942 genomic DNA | Synechoccocus elongatus PCC7942 genomic DNA | ||
Revision as of 03:14, 2 November 2017
Design Notes
PCR primers for backbone pPIGBACK (BBa_K2350009) to get Ampicillin resistance gene which has been processed a PstI site-directed mutagenesis
Forward: TGCGCTCTCGCTAGTGGTCAcgcggaacccctatttgttt
Reverse: CACCCGCTGCAAAGCACGATttaccaatgcttaatcagtg
PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase upstream
Forward: CAATATCCCGTAAAGTTTTT
Reverse: aaacaaataggggttccgcgTGACCACTAGCGAGAGCGCA
PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase downstream
Forward: cactgattaagcattggtaaATCGTGCTTTGCAGCGGGTG
Reverse: CGGCGTTACCGTCACGTAGT
NOTE:
In order to process a 3 pieces fusion PCR, the PCR primers for Lycopene cyclase upstream and downstream are different from the primers showed in BBa_K2350007 and BBa_K2350008.
Source
Synechoccocus elongatus PCC7942 genomic DNA
Backbone pPIGBACK (BBa_K2350009)