Difference between revisions of "Part:BBa K2346003"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2346003 short</partinfo> | <partinfo>BBa_K2346003 short</partinfo> | ||
| − | This is a composite part. The Pyear promoter will be switched on under the presence of nitrate or nitrite, translating P2 ogr. The P2 ogr will cause inducible PF promoter to produce even stronger downstream PoPS. We attached a RBS for convince of registry users. | + | This is a composite part and a functional improved device for Part:BBa_K216005(https://parts.igem.org/Part:BBa_K216005). The Pyear promoter will be switched on under the presence of nitrate or nitrite, translating P2 ogr. The P2 ogr will cause inducible PF promoter to produce even stronger downstream PoPS. We attached a RBS for convince of registry users. |
| + | We ligated this part with a GFP+terminator. | ||
| + | [[Image:BBa K2346003.png]] | ||
| + | Conclusion | ||
| + | As we can see from the graph, there is significant difference between NiSAP’s response to nitrate(40mM) and Pyear+GFP’s response to nitrate(40mM). After 3 hours in the plate reader, NiSAP’s A.U. is nearly twice the magnitude of Pyear+GFP. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 03:12, 2 November 2017
Nitrate/nitrite responsive promoter with amplifier
This is a composite part and a functional improved device for Part:BBa_K216005(https://parts.igem.org/Part:BBa_K216005). The Pyear promoter will be switched on under the presence of nitrate or nitrite, translating P2 ogr. The P2 ogr will cause inducible PF promoter to produce even stronger downstream PoPS. We attached a RBS for convince of registry users.
We ligated this part with a GFP+terminator.
Conclusion
As we can see from the graph, there is significant difference between NiSAP’s response to nitrate(40mM) and Pyear+GFP’s response to nitrate(40mM). After 3 hours in the plate reader, NiSAP’s A.U. is nearly twice the magnitude of Pyear+GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 508
- 1000COMPATIBLE WITH RFC[1000]