Difference between revisions of "Part:BBa K2448035:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
pPsiR At wt
 
−
 
  
 +
This biosensor was built using the Psicose Biosensor pPsiA-PsiR from ''Agrobacterium tumefaciens'' ([[Part:BBa_K2448025|BBa_K2448025]]) to which we added downstream the D-Psicose-3-Epimerase from ''C. cellulolyticum'' ([[Part:BBa_K2448021|BBa_K2448021]]).
  
 
===Source===
 
===Source===
  
−
pPsiR At wt
+
DNA synthesis of individual parts and assembly by Golden Gate.
  
 
===References===
 
===References===

Latest revision as of 03:12, 2 November 2017


Psicose Biosensor pPsiA-At PsiR-At with D-Psicose 3-epimerase from Clostridium cellulolyticum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1337
    Illegal XhoI site found at 62
    Illegal XhoI site found at 2618
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 104
    Illegal AgeI site found at 791
    Illegal AgeI site found at 2351
    Illegal AgeI site found at 2463
    Illegal AgeI site found at 3252
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biosensor was built using the Psicose Biosensor pPsiA-PsiR from Agrobacterium tumefaciens (BBa_K2448025) to which we added downstream the D-Psicose-3-Epimerase from C. cellulolyticum (BBa_K2448021).

Source

DNA synthesis of individual parts and assembly by Golden Gate.

References