Difference between revisions of "Part:BBa K2194002"

Revision as of 03:11, 2 November 2017

PgntK Negative Feedback Membrane Stress Promoter

The Pgntk promoter is a native Escherichia coli negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the gntK gene in Escherichia coli, which has been previously experimentally shown to incorporate the full Pgntk promoter region. [1]

Biology and Usage

PgntK is native promoter of the gluconate metabolism operon in E. coli. It helps mitigate stress imposed by the overexpression of membrane proteins by providing negative feedback regulation of the membrane protein genes. It has been demonstrated that PgntK can optimize expression levels of AcrB drug efflux pump to maximize the growth rate and minimize cellular toxicity [1].

Figure 1: PgntK (BBa_K2194002) Promoter similar strength to Pcon (BBa_J23108) Figure 1: we compared the reporter protein expression of E. coli MG1655 transformed with a PgntK promoter+mCherry construct versus a Pcon promoter+mCherry construct. The data indicates that PgntK promoter has a comparable transcription initiation rate to Pcon promoter when no membrane stress is present. [1] Boyarskiy, Sergey et al. “Transcriptional Feedback Regulation of Efflux Protein Expression for Increased Tolerance to and Production of N-Butanol.” Metabolic Engineering 33 (2016): 130–137. Web. 28 Oct. 2017. Sequence and Features