Difference between revisions of "Part:BBa K2350014:Design"
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Synechoccocus elongatus PCC7942 genomic DNA | Synechoccocus elongatus PCC7942 genomic DNA | ||
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Revision as of 03:10, 2 November 2017
Design Notes
PCR primers for backbone pPIGBACK (BBa_K2350009) to get Ampicillin resistance gene which has been processed a PstI site-directed mutagenesis
Forward: TGCGCTCTCGCTAGTGGTCAcgcggaacccctatttgttt
Reverse: CACCCGCTGCAAAGCACGATttaccaatgcttaatcagtg
PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase upstream
Forward:
Reverse:
PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase downstream
Forward:
Reverse:
NOTE: The PCR primers for Lycopene cyclase upstream and downstream are different from the primers showed in BBa_K2350007 and BBa_K2350008
Source
Synechoccocus elongatus PCC7942 genomic DNA
Backbone pPIGBACK (BBa_K2350009)