Difference between revisions of "Part:BBa K2195000:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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https://static.igem.org/mediawiki/2017/b/ba/Results01.png
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how you used this part and how it worked out.
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    <p style="font-size:1vw">The cells that were transformed to contain Dsup were found to have significantly higher survival rates than those without Dsup. Overall, cells containing Dsup--both induced and uninduced--showed an average survival rate of approximately 26% after four hours of exposure to UV-B radiation, while the cells containing only blue chromoprotein (without any radiation resistance genes) showed a survival rate of 0% after four hours. The most pronounced difference between cells containing Dsup and cells containing no radiation resistance was found at three hours, with Dsup-containing cells having an average survival rate of 49% and non-Dsup-containing cells having an average survival rate of 2.3%, meaning that cells with Dsup survived at a rate that was, on average, 2130% higher than the rate at which non-Dsup cells survived.
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https://static.igem.org/mediawiki/2017/9/9a/Results02.png
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    <p style="font-size:1vw">There was no significant difference observed in the survival rates of cells in which expression of Dsup was induced via addition of IPTG and cells in which it was not induced, indicating extremely leaky expression in the Lac promoter. </p>
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===Applications of BBa_K2195000===
 
===Applications of BBa_K2195000===

Latest revision as of 03:08, 2 November 2017


Results01.png

The cells that were transformed to contain Dsup were found to have significantly higher survival rates than those without Dsup. Overall, cells containing Dsup--both induced and uninduced--showed an average survival rate of approximately 26% after four hours of exposure to UV-B radiation, while the cells containing only blue chromoprotein (without any radiation resistance genes) showed a survival rate of 0% after four hours. The most pronounced difference between cells containing Dsup and cells containing no radiation resistance was found at three hours, with Dsup-containing cells having an average survival rate of 49% and non-Dsup-containing cells having an average survival rate of 2.3%, meaning that cells with Dsup survived at a rate that was, on average, 2130% higher than the rate at which non-Dsup cells survived.

Results02.png

There was no significant difference observed in the survival rates of cells in which expression of Dsup was induced via addition of IPTG and cells in which it was not induced, indicating extremely leaky expression in the Lac promoter. 

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Applications of BBa_K2195000

User Reviews

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