Latest revision as of 03:07, 2 November 2017

PhiC31 att B

The attB site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).

Reference: Amy C. Groth, Michele P. Calos, Phage Integrases: Biology and Applications, Journal of Molecular Biology, Volume 335, Issue 3, 16 January 2004, Pages 667-678, ISSN 0022-2836, http://dx.doi.org/10.1016/j.jmb.2003.09.082.

Improvement and Characterization by Fudan_China 2017

• Group: Fudan_China 2017
• Author: Haiyun Liu, Fudan University

We improved this part and characterized the kinetic characterization and the orthogonality between phiC31 and some other serine recombinase using this improved attB site (BBa_K2460010). For more information, go to phiC31 integrase.

Sequence and Features