Difference between revisions of "Part:BBa K912001"
Ykishimotoy (Talk | contribs) |
Ykishimotoy (Talk | contribs) |
||
| Line 12: | Line 12: | ||
190μl CH3COOH Buffer | 190μl CH3COOH Buffer | ||
| − | Digest phytic acid in above situation with sample phytase solution 10μl. | + | Digest phytic acid in above situation with sample phytase solution 10μl ,37degrees celcius, 10 min. |
After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes. | After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes. | ||
Revision as of 03:07, 2 November 2017
E. coli phytase (appa phytase)
Part to produce the appa e. coli phytase. This phytase acts to break down phytic acid into inositol, releasing chelated iron and other micronutrients.
HokkaidoU_Japan 2017 analyzed this part. This protein's activity falls as below graph.
Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer
Digest phytic acid in above situation with sample phytase solution 10μl ,37degrees celcius, 10 min.
After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 688
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1213