Difference between revisions of "Part:BBa K912001"
Line 7: Line 7:
  
 
HokkaidoU_Japan 2017 analyzed this part. This protein's activity falls as below graph.
 
HokkaidoU_Japan 2017 analyzed this part. This protein's activity falls as below graph.
 +
 +
Protocol:
 +
50μl 7.5 mM Phytic acid
 +
190μl CH3COOH Buffer
 +
 +
Digest phytic acid in above situation with sample phytase solution 10μl.
 +
 +
After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes.
  
 
https://static.igem.org/mediawiki/2017/9/95/T--HokkaidoU_Japan--K912001gurafu.png
 
https://static.igem.org/mediawiki/2017/9/95/T--HokkaidoU_Japan--K912001gurafu.png

Revision as of 03:06, 2 November 2017


E. coli phytase (appa phytase)

Part to produce the appa e. coli phytase. This phytase acts to break down phytic acid into inositol, releasing chelated iron and other micronutrients.


HokkaidoU_Japan 2017 analyzed this part. This protein's activity falls as below graph.

Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer

Digest phytic acid in above situation with sample phytase solution 10μl.

After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes.

T--HokkaidoU_Japan--K912001gurafu.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1213