Difference between revisions of "Part:BBa K2350014:Design"

 
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===Design Notes===
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PCR primers for backbone pPIGBACK (BBa_K2350009) to get Ampicillin resistance gene which has been processed a PstI site-directed mutagenesis
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<br>
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Forward: TGCGCTCTCGCTAGTGGTCAcgcggaacccctatttgttt
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<br>
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Reverse: CACCCGCTGCAAAGCACGATttaccaatgcttaatcagtg
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<br>
  
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PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase upstream
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<br>
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Forward:
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<br>
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Reverse:
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<br>
 +
 +
PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase downstream
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<br>
 +
Forward:
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<br>
 +
Reverse:
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 +
<br>
 +
NOTE: The PCR primers for Lycopene cyclase upstream and downstream are different from the primers showed in BBa_K2350007 and BBa_K2350008

Revision as of 03:05, 2 November 2017

Design Notes

PCR primers for backbone pPIGBACK (BBa_K2350009) to get Ampicillin resistance gene which has been processed a PstI site-directed mutagenesis
Forward: TGCGCTCTCGCTAGTGGTCAcgcggaacccctatttgttt
Reverse: CACCCGCTGCAAAGCACGATttaccaatgcttaatcagtg

PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase upstream
Forward:
Reverse:

PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase downstream
Forward:
Reverse:


NOTE: The PCR primers for Lycopene cyclase upstream and downstream are different from the primers showed in BBa_K2350007 and BBa_K2350008