Difference between revisions of "Part:BBa K2194003"
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<p class = "caption"> <b> Figure 1: Effect of Chromate on <i>E. coli</i> Growth </b> </p> | <p class = "caption"> <b> Figure 1: Effect of Chromate on <i>E. coli</i> Growth </b> </p> | ||
Revision as of 02:59, 2 November 2017
cysPUWA Sulfate Transport System cds
The cysPUWA genes encode the sulfate ABC membrane transporter in Escherichia coli. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for cysP, cysU, cysW, and cysA in the context in which they appear in E. coli MG1655.
Biology and Usage
Cys P is a binding protein which increases the affinity for sulfate uptake; genes cysU, cysW encode the integral membrane proteins. cysA is an ATP-binding subunit.
Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI): lower growth rate for cysPUWA transformants in the presence of Cr(VI) may indicate the higher rate of chromium transport inside the cell.
Figure 1: Effect of Chromate on E. coli Growth
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1274
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2090
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal BsaI.rc site found at 3828