Difference between revisions of "Part:BBa K2442400"

 
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This is a composite of the GFP C-term split subunit (GFP2) [https://parts.igem.org/Part:BBa_K2442397 K2442397] and the well characterised "weak" ribosome binding site [https://parts.igem.org/Part:BBa_B0032 B0032].
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===Usage and Biology===
 
===Usage and Biology===
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Despite the sequence error in the original [https://parts.igem.org/Part:BBa_K2442397 K2442397] part, this composite was used for assembly of larger composite split-GFP AND-gate constructs.
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*[https://parts.igem.org/Part:BBa_K2442406 K2442406] [split GFP module 2] lacI regulated promoter + RBS + GFP C-term + double terminator
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*[https://parts.igem.org/Part:BBa_K2442408 K2442408] Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]
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Revision as of 02:59, 2 November 2017


B0032 + GFP split C-term

This is a composite of the GFP C-term split subunit (GFP2) K2442397 and the well characterised "weak" ribosome binding site B0032.


Usage and Biology

Despite the sequence error in the original K2442397 part, this composite was used for assembly of larger composite split-GFP AND-gate constructs.

  • K2442406 [split GFP module 2] lacI regulated promoter + RBS + GFP C-term + double terminator
  • K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 195