Difference between revisions of "Part:BBa K2471014"

Latest revision as of 02:58, 2 November 2017

glycosyltransferase family 2 protein [Bacillus subtilis]

This gene producesa protein that arrests flagellar rotation in a similar way to that of a clutch, this by disengaging motor force-generating elements in cells embedded in the biofilm matrix. The clutch is a simple, rapid, and potentially reversible form of motility control. This gene is sufficient to inhibit motility and does so by arresting flagellar rotation (Blair, K. M., 2008). Though the EPS operon is normally repressed in B. subtilis, it's beneficial for the original copy of the gene to be knocked out if its synthetically expressed. Although many bacterial flagellar assemblies contain proteins that are similar in shape, there is no guarantee that the epsE gene will function correctly in any host cell other than B. subtilis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2471014 short</partinfo>
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-the epsE enzyme, which has been suggested to function in a manner similar to a molecular clutch. EpsE could potentially be used as a controller of bacterial movement, promoting biofilm formation and inhibiting motility. Though the EPS operon is normally repressed in B. subtilis, it is beneficial for the original copy of epsE gene to be knocked out if EpsE is synthetically expressed. Although many bacterial flagellar assemblies contain proteins that are similar in shape, there is no guarantee that the epsE gene will function correctly in any host cell other than B. subtilis.
+This gene producesa protein that arrests flagellar rotation in a similar way to that of a clutch, this by disengaging motor force-generating elements in cells embedded in the biofilm matrix. The clutch is a simple, rapid, and potentially reversible form of motility control. This gene is sufficient to inhibit motility and does so by arresting flagellar rotation (Blair, K. M., 2008). Though the EPS operon is normally repressed in <i>B. subtilis</i>, it's beneficial for the original copy of the gene to be knocked out if its synthetically expressed. Although many bacterial flagellar assemblies contain proteins that are similar in shape, there is no guarantee that the epsE gene will function correctly in any host cell other than <i>B. subtilis</i>.
+<br><br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===