Difference between revisions of "Part:BBa K2460010"

Latest revision as of 02:58, 2 November 2017

φC31 attB

This part is improved from BBa_K1039035.

This is a recombination site attB for phiC31 integrase (BBa_K1039012). When used with phiC31 attP (BBa_K2460006) and phiC31 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.

For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiC31 integrase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This part is improved from [[Part:BBa_K1039035|BBa_K1039035]].
 This is a recombination site attB for phiC31 integrase ([[Part:BBa_K1039012|BBa_K1039012]]). When used with phiC31 attP ([[Part:BBa_K2460006|BBa_K2460006]]) and phiC31 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.
 For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to [[Part:BBa_K1039012|phiC31 integrase]].