Difference between revisions of "Part:BBa K2364027"
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2364027 parameters</partinfo>
 
<partinfo>BBa_K2364027 parameters</partinfo>
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<img src='https://static.igem.org/mediawiki/2017/3/3b/Photo_3.jpeg' />
 

Revision as of 02:55, 2 November 2017


pCMV-DsRed-T145-T143-TFF4-SV40 polyA

DsRed expressed under the constitutive promoter CMV. Binding sites for the miR-145-5p, miR-143-3p and the synthetic miRNA FF4 are fused in the 3'-untranslated region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters