Difference between revisions of "Part:BBa K2195000"
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This protein is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species <i>Ramazzottius Varieornatus</i>. It was found to be a protein that protects tardigrades from X-ray damage and is currently believed to be a DNA binding protein. Since only X-ray damage was tested, we decided to test if Dsup can protect organisms from UV radiation as well since it was not theorized to be a repair mechanism. | This protein is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species <i>Ramazzottius Varieornatus</i>. It was found to be a protein that protects tardigrades from X-ray damage and is currently believed to be a DNA binding protein. Since only X-ray damage was tested, we decided to test if Dsup can protect organisms from UV radiation as well since it was not theorized to be a repair mechanism. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
We found that Dsup can protect DH5α E. coli from UV-B radiation that normally causes pyrimidine dimers in the DNA. Using our UV irradiation protocol, we found that E. coli cells with the Dsup gene had a lower death rate/higher survival rate than our control cells. | We found that Dsup can protect DH5α E. coli from UV-B radiation that normally causes pyrimidine dimers in the DNA. Using our UV irradiation protocol, we found that E. coli cells with the Dsup gene had a lower death rate/higher survival rate than our control cells. | ||
Revision as of 02:49, 2 November 2017
Dsup (Radiation Protection)
This protein is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species Ramazzottius Varieornatus. It was found to be a protein that protects tardigrades from X-ray damage and is currently believed to be a DNA binding protein. Since only X-ray damage was tested, we decided to test if Dsup can protect organisms from UV radiation as well since it was not theorized to be a repair mechanism.
Usage and Biology
We found that Dsup can protect DH5α E. coli from UV-B radiation that normally causes pyrimidine dimers in the DNA. Using our UV irradiation protocol, we found that E. coli cells with the Dsup gene had a lower death rate/higher survival rate than our control cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 240
Illegal BamHI site found at 694 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]