Difference between revisions of "Part:BBa K2292006:Design"

 
 
Line 12: Line 12:
  
 
===Source===
 
===Source===
 +
We crafted our gene from sequences of mazE & mazF found in NCBI
  
−
1. Escherichia coli mazEF-Mediated Cell Death Is Triggered by Various Stressful Conditions
 
  
−
2. Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding
+
===References===
  
−
3. mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria
+
1. PMC415763 - Escherichia coli mazEF-Mediated Cell Death Is Triggered by Various Stressful Conditions
  
 +
2.  PMC4333400 -  Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding
  
−
===References===
+
3.  doi: 10.1242/jcs.02619 - mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria

Latest revision as of 02:41, 2 November 2017


MazEF kill switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

n/a


Source

We crafted our gene from sequences of mazE & mazF found in NCBI


References

1. PMC415763 - Escherichia coli mazEF-Mediated Cell Death Is Triggered by Various Stressful Conditions

2. PMC4333400 - Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding

3. doi: 10.1242/jcs.02619 - mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria