Difference between revisions of "Part:BBa K2522000"

Latest revision as of 02:27, 2 November 2017

csgA-dCBD (Curli fibers attachable to cellulose)

The plasmid created has a protein subunit with a cellulose binding domain at the end in order to allow an E. coli cell to encase itself in cellulose. Curli are hairlike surface proteins that cover E. coli, permitting the bacteria to adhere to surfaces. The CsgA subunits are the building blocks that make up the fibrous section of curli. If a cellulose binding domain could be attached to each CsgA subunit, the cell could encapsulate itself in cellulose. This characteristic would render the cell resistant to digestion in a human GI tract.

NOTE: This part is an improvement on Part BBa_K1321340, just the dCBD by itself.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 18:54, 17 September 2017 (view source) Registry (Talk \| contribs)		Latest revision as of 02:27, 2 November 2017 (view source) Dmcdonald17 (Talk \| contribs)
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	The plasmid created has a protein subunit with a cellulose binding domain at the end in order to allow an E. coli cell to encase itself in cellulose. Curli are hairlike surface proteins that cover E. coli, permitting the bacteria to adhere to surfaces. The CsgA subunits are the building blocks that make up the fibrous section of curli. If a cellulose binding domain could be attached to each CsgA subunit, the cell could encapsulate itself in cellulose. This characteristic would render the cell resistant to digestion in a human GI tract.		The plasmid created has a protein subunit with a cellulose binding domain at the end in order to allow an E. coli cell to encase itself in cellulose. Curli are hairlike surface proteins that cover E. coli, permitting the bacteria to adhere to surfaces. The CsgA subunits are the building blocks that make up the fibrous section of curli. If a cellulose binding domain could be attached to each CsgA subunit, the cell could encapsulate itself in cellulose. This characteristic would render the cell resistant to digestion in a human GI tract.
		+
		+	NOTE: This part is an improvement on Part BBa_K1321340, just the dCBD by itself.