Difference between revisions of "Part:BBa K2194004"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2194004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2194004 SequenceAndFeatures</partinfo>
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===Source===
 
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See the registry pages for negative feedback membrane stress promoter <i>PgntK</i> ([https://parts.igem.org/Part:BBa_K2194002  BBa_K2194002]), cysPUWA proteins ([https://parts.igem.org/Part:BBa_K2194003  BBa_K2194003]), Elowitz RBS ([https://parts.igem.org/Part:BBa_B0034  BBa_B0034]), and sulfate binding protein sbp ([https://parts.igem.org/Part:BBa_K2194001  BBa_K2194001]) sources.
 
  
  

Revision as of 02:08, 2 November 2017


Sulfate Transport System w/ Negative Feedback for Membrane Stress

This transcriptional unit codes for the genetic parts necessary to bind and transport chromate, sulfate, and related species across the cell membrane. BioBrick BBa_K2194004 is a composite part composed of negative feedback membrane stress promoter PgntK (BBa_K2194002), a synthetic RBS, cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), sulfate binding protein sbp (BBa_K2194001), and a synthetic terminator.

Biology and Usage

Refer to the registry pages for PgntK (BBa_K2194002), a synthetic RBS, cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), sulfate binding protein sbp (BBa_K2194001) for detailed biology.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2296
  • 1000
    COMPATIBLE WITH RFC[1000]