Difference between revisions of "Part:BBa K2384013"
 
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<partinfo>BBa_K2384013 short</partinfo>
 
<partinfo>BBa_K2384013 short</partinfo>
  
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<p>Xylose-inducible expression system: strong<b> P<i>xylA</i></b> promoter and repressor gene <b><i>xylR</i></b>; after xylose addition, the repressor is released from the P<i>xylA</i> that activates transcription initiation.([[Part:BBa_K733002|BBa_K733002]]) They have been designed for high expression in <i>B. megaterium</i> This part serves as a functional MT for heavy metal binding and conferred resistance toxic metal cadmium. GST as a fusion partenr is to enhance stability of expression.The original part was from Cornell([[Part:BBa_K1460002|BBa_K1460002]]). This year our parts were doing codon optimisation but without terminator codon.</p>
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<p>Xylose-inducible expression system: strong<b> P<i>xylA</i></b> promoter and repressor gene <b><i>xylR</i></b>; after xylose addition, the repressor is released from the P<i>xylA</i> that activates transcription initiation.([[Part:BBa_K733002|BBa_K733002]]) They have been designed for high expression in <i>B. megaterium</i>. This part serves as a functional MT for heavy metal binding and conferred resistance toxic metal cadmium. GST as a fusion partenr is to enhance stability of expression.The original part was from Cornell([[Part:BBa_K1460002|BBa_K1460002]]). This year our parts were doing codon optimisation but without terminator codon.</p>
  
  

Latest revision as of 02:01, 2 November 2017


Repressor gene xylR+Strong PxylA promoter+RBS+GST-CRS5+His-tag

Xylose-inducible expression system: strong PxylA promoter and repressor gene xylR; after xylose addition, the repressor is released from the PxylA that activates transcription initiation.(BBa_K733002) They have been designed for high expression in B. megaterium. This part serves as a functional MT for heavy metal binding and conferred resistance toxic metal cadmium. GST as a fusion partenr is to enhance stability of expression.The original part was from Cornell(BBa_K1460002). This year our parts were doing codon optimisation but without terminator codon.


Usage and Biology

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.

Figure 1:After Codon Adjustment


2.GC Content: The comparison of GC content between original sequence and optimized sequence

Figure 2:After Optimization
Figure 3:The expression of GST-CRS5 detected using SDS-PAGE
Figure 4:Plasmid digested by EcoRI and HindIII
Figure 5:Our genetic circuit

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2159
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1571