Difference between revisions of "Part:BBa K2381001"
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<partinfo>BBa_K2381001 short</partinfo>
 
<partinfo>BBa_K2381001 short</partinfo>
  
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<p>nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms. <br/>
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<p>nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm<sup>2</sup>). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms. <br/>
 
This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts <partinfo>BBa_K2381019.</partinfo> <br/>
 
This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts <partinfo>BBa_K2381019.</partinfo> <br/>
 
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.
 
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.
 
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 01:57, 2 November 2017


nMagHigh


nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms.
This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts BBa_K2381019.
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403