Difference between revisions of "Part:BBa K2457002:Design"

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<partinfo>BBa_K2457002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2457002 SequenceAndFeatures</partinfo>
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===References===
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Chen, Y. J., Liu, P., Nielsen, A. A., Brophy, J. A., Clancy, K., Peterson, T., & Voigt, C. A. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nature methods, 2013.
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Dang, Ying et al. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome biology, 2015.
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Deltcheva, Elitza et al. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature, 2011.
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Farboud, B., & Meyer, B. J. Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Genetics, 2015.
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Jinek, Martin et al. A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity. Science, 2012.

Latest revision as of 01:54, 2 November 2017


Optimized sgRNA targeting LacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Chen, Y. J., Liu, P., Nielsen, A. A., Brophy, J. A., Clancy, K., Peterson, T., & Voigt, C. A. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nature methods, 2013.

Dang, Ying et al. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome biology, 2015.

Deltcheva, Elitza et al. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature, 2011.

Farboud, B., & Meyer, B. J. Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Genetics, 2015.

Jinek, Martin et al. A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity. Science, 2012.