Difference between revisions of "Part:BBa K2253002:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The coding sequence of the optimized blue chromoprotein had to be ligated into a vector containing a promoter and RBS sequence K608002.  
+
The codon-optimized blue chromoprotein was ligated into the BBa_K608002 vector containing a strong promoter and RBS sequence to form a basic part.  
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The original blue chromoprotein sequence comes from coral Acropora millepora, mail CP and was retrieved from the 2011 iGEM Uppsala-Sweden team after which it was codon optimized using Interactive DNA Technologies.  
+
The original blue chromoprotein sequence comes from the coral <i>Acropora millepora</i>. The blue chromoprotein part was retrieved from the 2011 iGEM Uppsala-Sweden team and was subsequently codon-optimized using the Codon Optimization tool provided by Interactive DNA Technologies.  
  
 
===References===
 
===References===

Latest revision as of 01:46, 2 November 2017


Optimized Blue Chromoprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 694
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon-optimized blue chromoprotein was ligated into the BBa_K608002 vector containing a strong promoter and RBS sequence to form a basic part.


Source

The original blue chromoprotein sequence comes from the coral Acropora millepora. The blue chromoprotein part was retrieved from the 2011 iGEM Uppsala-Sweden team and was subsequently codon-optimized using the Codon Optimization tool provided by Interactive DNA Technologies.

References