Difference between revisions of "Part:BBa K2365517"
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===Under testing=== | ===Under testing=== | ||
| − | [[File: | + | [[File:JUB1444.png|400px|center]] |
[[File:JUB1.jpg|400px|center]] | [[File:JUB1.jpg|400px|center]] | ||
To establish orthogonal regulators for use in yeast (Saccharomyces cerevisiae), we chose TFs from the plant Arabidopsis thaliana. We established a GFP green -based reporter system to test the effect of plant regulators in the yeast background. | To establish orthogonal regulators for use in yeast (Saccharomyces cerevisiae), we chose TFs from the plant Arabidopsis thaliana. We established a GFP green -based reporter system to test the effect of plant regulators in the yeast background. | ||
Latest revision as of 01:25, 2 November 2017
JUB1 promoter
Arabidopsis NAC TFs JUNGBRUNNEN1 (JUB1)-------promoter (3*UAS)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 327
Illegal BamHI site found at 432 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4
Under testing
To establish orthogonal regulators for use in yeast (Saccharomyces cerevisiae), we chose TFs from the plant Arabidopsis thaliana. We established a GFP green -based reporter system to test the effect of plant regulators in the yeast background.