Difference between revisions of "Part:BBa K2378009"
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<partinfo>BBa_K2378009 short</partinfo> | <partinfo>BBa_K2378009 short</partinfo> | ||
| − | This is coding device of | + | This is coding device of EndA DNA-specific endonuclease, with constitutive promoter BBa_J61051. The gene is isolated from <i>Vibrio cholera</i>. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <p>EndA endonuclease is expressed entirely extracellularly. Its natural function in nature is to prevent transformation when expressed, so that <i>Vibrio cholera</i> can manage the period and seasons for transformation.</p> | ||
| + | <p>Utilizing that characteristics, this enzyme can be used to coat bacterial cells in endonuclease cloud. This can prevent natural transformation from happening. This function is particularly useful as a containment/safety device to prevent transformation of natural organisms by genetically modified ones which can disrupt the natural gene pool. </p> | ||
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Revision as of 01:22, 2 November 2017
EndA Endonuclease Coding Device from Vibrio cholera
This is coding device of EndA DNA-specific endonuclease, with constitutive promoter BBa_J61051. The gene is isolated from Vibrio cholera.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 305
Illegal NgoMIV site found at 545 - 1000COMPATIBLE WITH RFC[1000]