Difference between revisions of "Part:BBa K2319007"
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<partinfo>BBa_K2319007 short</partinfo> | <partinfo>BBa_K2319007 short</partinfo> | ||
| − | This part is an improved BBa_J18932 (mCherry RFP), created by modifying the sequence preceding the hidden translational start site near the N-terminus | + | This part is an improved BBa_J18932 (mCherry RFP), created by modifying the sequence preceding the hidden translational start site near the N-terminus. This weakens the RBS and reduces translation initiation at the internal ATG, thereby reducing the percentage of truncated protein. |
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Latest revision as of 01:14, 2 November 2017
improved mCherry RFP
This part is an improved BBa_J18932 (mCherry RFP), created by modifying the sequence preceding the hidden translational start site near the N-terminus. This weakens the RBS and reduces translation initiation at the internal ATG, thereby reducing the percentage of truncated protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]