Difference between revisions of "Part:BBa K2475001"

Revision as of 01:10, 2 November 2017

Sup 35 Prion Domain fused to Cyan Fluorescent Protein (PrD-CFP)

This composite part consists of the Sup35 aggregation domain fused with CFP (cyan fluorescent protein), which is a fluorescent tag with a theoretical excitation peak at 430 nm and emission peak at 485 nm. This was used, in conjunction with PrD-YFP, in Förster resonance energy transfer (FRET) experiments to test if/how the aggregation domain affects the energy transfer from CFP to YFP in PSI+ (aggregate state) and psi- (soluble state).

Sup35 aggregation domain is from BBa_K2475000

Used with BBa_K2475002 to collect FRET data.

Sequence and Features