Difference between revisions of "Part:BBa K2475001"

Revision as of 01:09, 2 November 2017

Sup 35 Prion Domain fused to Cyan Fluorescent Protein (PrD-CFP)

This composite part consists of the Sup35 aggregation domain fused with CFP (cyan fluorescent protein), which is a fluorescent tag with a theoretical excitation peak at 430 nm and emission peak at 485 nm. This was used, in conjunction with PrD-YFP, in Förster resonance energy transfer (FRET) experiments to test if/how the aggregation domain affects the energy transfer from CFP to YFP in PSI+ (aggregate state) and psi- (soluble state).

Sup35 aggregation domain is from BBa_K2475000 Used with BBa_K2475002 to collect FRET data.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 715
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This composite part consists of the Sup35 aggregation domain fused with CFP (cyan fluorescent protein), which is a fluorescent tag with a theoretical excitation peak at 430 nm and emission peak at 485 nm. This was used, in conjunction with PrD-YFP, in Förster resonance energy transfer (FRET)  experiments  to  test if/how the aggregation domain affects the energy transfer from CFP to YFP in PSI+ (aggregate state) and psi- (soluble state).
+Sup35 aggregation domain is from <partinfo>BBa_K2475000</partinfo>
 Used with <partinfo>BBa_K2475002</partinfo> to collect FRET data.
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