Difference between revisions of "Part:BBa K2486007"

Latest revision as of 00:58, 2 November 2017

lldR repressor generator

A part that constitutively generates the LldR repressor

This part consists of:

A weak promoter from Anderson's Promoter Library (BBa_J23114): You don't want a lot of repressor protein when you design a biosensor, this would make the biosensor less sensitive, so this promoter is adequate for use in biosensor circuits.
An RBS based on Elowitz repressilator (BBa_B0034).
The regulatory protein LldR (BBa_K1847001) which represses ldhA and lldD, genes that encode for lactate dehydrogenates.
A terminator

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 874
Illegal NheI site found at 897
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 284
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 This part consists of:
-- A weak promoter from Anderson's Promoter Library (BBa_J23114): You don't want a lot of repressor protein when you design a biosensor, this would make the biosensor less sensitive, so this promoter is adequate for use in biosensor circuits.
+# A weak promoter from Anderson's Promoter Library (BBa_J23114): You don't want a lot of repressor protein when you design a biosensor, this would make the biosensor less sensitive, so this promoter is adequate for use in biosensor circuits.
-- An RBS based on Elowitz repressilator (BBa_B0034).
+# An RBS based on Elowitz repressilator (BBa_B0034).
-- The regulatory protein LldR (BBa_K1847001) which represses ldhA and lldD, genes that encode for lactate dehydrogenates.
+# The regulatory protein LldR (BBa_K1847001) which represses ldhA and lldD, genes that encode for lactate dehydrogenates.
-- A terminator
+# A terminator
 <span class='h3bb'>Sequence and Features</span>