Difference between revisions of "Part:BBa K2455005"
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2455005 short</partinfo>
 
<partinfo>BBa_K2455005 short</partinfo>
−
 
−
<b>DISCLAIMER:</b> This biobrick carries an illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region - For data and design please see [[Part:BBa_K2455002|R9-SYFP2-H6]].
 
  
 
This is an improved version of the Blue Fluorescent Protein (mTag BFP; [[Part:BBa_K592100|BBa_K592100]]) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving its associated protein the ability to pass through plasma membranes.
 
This is an improved version of the Blue Fluorescent Protein (mTag BFP; [[Part:BBa_K592100|BBa_K592100]]) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving its associated protein the ability to pass through plasma membranes.
Line 10: Line 8:
  
 
Previous studies have shown R9 associated proteins to be able to enter a variety of cell types ([https://academic.oup.com/pcp/article/46/3/482/1817347 Chang <i>et al., 2005</i>]).
 
Previous studies have shown R9 associated proteins to be able to enter a variety of cell types ([https://academic.oup.com/pcp/article/46/3/482/1817347 Chang <i>et al., 2005</i>]).
 +
 +
 +
<b>DISCLAIMER:</b> This biobrick carries an illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region - For data and design please see [[Part:BBa_K2455002|R9-SYFP2-H6]].
 +
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 00:54, 2 November 2017


CPP-mTag BFP

This is an improved version of the Blue Fluorescent Protein (mTag BFP; BBa_K592100) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving its associated protein the ability to pass through plasma membranes.

For our study we demonstrated that this improved version of mTag BFP had fluorescence of similar levels to the original protein, and was able to stain both Escherichia coli and Pseudomonas putida cells, presumably through internalisation of the protein.

Previous studies have shown R9 associated proteins to be able to enter a variety of cell types (Chang et al., 2005).


DISCLAIMER: This biobrick carries an illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region - For data and design please see R9-SYFP2-H6.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 741
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 741
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 741
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 741
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 741
  • 1000
    COMPATIBLE WITH RFC[1000]