Difference between revisions of "Part:BBa I737020"
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<partinfo>BBa_I737020 short</partinfo>
 
<partinfo>BBa_I737020 short</partinfo>
  
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This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. We then cloned it into pSB1A2 after amplifying with primers that add the BioBrick prefix and suffix. Currently the gene has a EcorRI and a PstI site which limits how it can be assembled.
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This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. We then cloned it into pSB1A2 using XbaI and SpeI after amplifying with primers that add the BioBrick prefix and suffix. Currently the gene has a EcorRI and a PstI site which limits how it can be assembled.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:26, 26 October 2007

PoPS->Agarase (Digests agar)

This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. We then cloned it into pSB1A2 using XbaI and SpeI after amplifying with primers that add the BioBrick prefix and suffix. Currently the gene has a EcorRI and a PstI site which limits how it can be assembled.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1861
    Illegal NheI site found at 106
    Illegal NheI site found at 901
    Illegal PstI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1861
    Illegal XhoI site found at 1964
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
    Illegal AgeI site found at 368
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1319
    Illegal SapI.rc site found at 1717
    Illegal SapI.rc site found at 1795